Microassay for Human and Chick Cell Interferon
نویسندگان
چکیده
منابع مشابه
Microassay for human and chick cell interferons.
The microassay for human and chick interferon described in this paper required much smaller amounts of samples and reagents and considerably less time and effort than the plaque reduction assay while yielding comparable results.
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Kreuz, Leo E. (The Johns Hopkins University School of Medicine, Baltimore, Md.), and Allan H. Levy. Physical properties of chick interferon. J. Bacteriol. 89:462-469. 1965.-The sedimentation coefficient, diffusion coefficient, and molecular weight of chick-embryo interferon were determined by zone centrifugation and equilibrium sedimentation in cesium chloride density gradients, and by chromato...
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A microcytotoxic assay, based on the release of 51Cr from chicken erythrocytes by sensitized chicken lymphoid cells in the presence of the specific antigen, purified protein derivative (PPD), is described. The percentage of antigen-specific lysis was dependent on the lymphoid cell concentration, antigen concentration, time of incubation, and incubation temperature. Maximum antigen-specific lysi...
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Background: Interferon α-2b is a vital biotherapeutic produced through the recombinant DNA technology in E. coli. The recombinant IFN-α2b normally appears as intercellular IBs, which requires intensive refolding and purification steps. Method: Purification of IFN-α2b from solubilized IB was performed using two-phase extraction. To optimize refolding conditions, the effects of pH and different a...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1968
ISSN: 0003-6919
DOI: 10.1128/aem.16.11.1706-1707.1968